p egfr py 1068 Search Results


rabbit anti p egfr  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti p egfr
Rabbit Anti P Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phosphorylated p egfr antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti phosphorylated p egfr antibody
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Rabbit Anti Phosphorylated P Egfr Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfr p  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc egfr p
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Egfr P, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho egfr tyr1068  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology phospho egfr tyr1068
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Phospho Egfr Tyr1068, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfr py-1068 antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc egfr py-1068 antibody
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Egfr Py 1068 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p egfr tyr1068  (Cell Signaling Technology Inc)
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Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Anti P Egfr Tyr1068, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p egfr tyr1068  (Cell Signaling Technology Inc)
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Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
P Egfr Tyr1068, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti tyr 1068 p egfr  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti tyr 1068 p egfr
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Rabbit Anti Tyr 1068 P Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p egfr  (Proteintech)
96
Proteintech p egfr
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
P Egfr, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p egfr  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc p egfr
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
P Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phosphotyrosine egfr  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti phosphotyrosine egfr
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Rabbit Anti Phosphotyrosine Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-perk antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-perk antibody
Figure 2. FLNa knockdown promotes EGF‑induced <t>EGFR/Akt/ERK</t> phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, <t>phosphorylated.</t>
Anti Perk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. FLNa knockdown promotes EGF‑induced EGFR/Akt/ERK phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, phosphorylated.

Journal: Molecular medicine reports

Article Title: Filamin A regulates EGFR/ERK/Akt signaling and affects colorectal cancer cell growth and migration.

doi: 10.3892/mmr.2019.10622

Figure Lengend Snippet: Figure 2. FLNa knockdown promotes EGF‑induced EGFR/Akt/ERK phosphorylation in SW480 cells. SW480 cells were transfected with shFLNa or control shRNA for 24 h. Then cells were treated with or without EGF (20 nM) for 0, 5, 10 and 30 min. (A) Total proteins were extracted and subjected to western blotting with antibodies against FLNa, EGFR, p‑EGFR, p‑Akt, Akt, p‑ERK and ERK; β‑actin is shown here as a loading control. (B) Band intensities for the ratio of FLN/β‑actin were measured. (C) Band intensities for the ratio of p‑EGFR/EGFR, (D) p‑Akt/Akt and (E) p‑ERK/ERK were measured and normalized to β‑actin. **P<0.01 vs. SW480/Ctrl. FLNa, Filamin A; EGF, epidermal growth factor; sh, short hairpin; EGFR, epidermal growth factor receptor; p‑, phosphorylated.

Article Snippet: Primary antibodies included the following: rabbit anti-Flna antibody (1:1,000; cat. no. MaB1680; eMd Millipore), rabbit anti-eGFr antibody (1:1,000; cat. no. 18986-1-aP; ProteinTech Group, inc.), rabbit anti-phosphorylated (p-)eGFr antibody (Tyr1068; 1:2,000; cat. no. 3777; cell Signaling Technology, inc.), rabbit anti-akt antibody (1:1,000; cat. no. 10176-2-aP; ProteinTech Group, inc.), rabbit anti-p-akt antibody (Ser473; 1:1,000; cat. no. 66444-1-ig; ProteinTech Group, inc.), rabbit anti-erK1/2 antibody (1:4,000; cat. no. 16443-1-aP; ProteinTech Group, inc.), rabbit anti-p-erK1/2 antibody (Thr202/Tyr204; 1:3,000; cat. no. 4370, cell Signaling Technology.) and mouse anti-β-actin antibody (1:5,000; cat. no. 60008-1-ig; ProteinTech Group, inc.).

Techniques: Knockdown, Phospho-proteomics, Transfection, Control, shRNA, Western Blot